Direct Quantitative Bisulfite Sequencing Using Tag-modified Primers and Internal Normalization
نویسندگان
چکیده
منابع مشابه
Direct sequencing of PCR products using unlabeled primers.
An improved protocol is described for using lambda exonuclease to directly sequence PCR products. It is important not to execute PCR cycles beyond the plateau of amplification. The asymmetric PCR and double-stranded DNA sequencing by a snap-cooling procedure were also performed using the same DNA samples and primers. The improved method was the most reliable and produced the best results.
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ژورنال
عنوان ژورنال: Anticancer Research
سال: 2016
ISSN: 0250-7005,1791-7530
DOI: 10.21873/anticanres.11231